In our previous article, A Deep Dive Into Ozonated Water, we ran a series of tests to determine how much ozone water can hold at different concentrations. Now we expand our testing to larger amounts of water to show how to best utilize ozone equipment and large bubblers more efficiently.

Why do we need a high ozone concentration?

The general consensus is that an ozonated water concentration above 4 ppm is considered therapeutic and is widely accepted as the concentration needed in dental or clinical settings. Even concentrations close to 4 ppm are considered adequate.

Even though most bacteria and viruses are neutralized at lower concentrations it’s important to achieve high ozonated water concentration to compensate for the half-life of ozone in water. The stronger the ozone concentration in the water the longer you can store it between uses. If you are able to refrigerate ozonated water it will maintain a higher concentration for a longer period of time.

How to get a high ozone concentration in water

This information should hopefully give people who are ozonating 1-2 liters of water a much better idea of what the ozone concentration in the water should be. This is very dependent on what the flow rate of oxygen is, the ozone concentration, and also the volume of water being ozonated.

Oxygen flow rates of ⅛ and 1/16 LPM are pretty close in terms of how much ozone is dissolved into the solution, so either of those flow rates will be ideal. Whichever generator you are using, be sure to maximize the output to the highest dial or power setting on the generator. This will help saturate the ozone into solution much faster than a lower concentration dial or power setting.

The water used in our testing was distilled (0 TDS) at room temperature (70-71°F). The bubbler system was an Erlenmeyer flask with a medium course glass diffuser stone. We’ve included the relative information below for reference.

Using 1000ml distilled water we tested the concentration in the water at both 10 and 20 minute increments:

1/2 LPM flow rate – 32 gamma
10 minutes — 4.7 ppm, 20 minutes — 5.2 ppm

¼ LPM flow rate – 56 gamma
10 minutes — 5.4 ppm, 20 minutes — 6.6 ppm

⅛ LPM flow rate – 75 gamma
10 minutes — 6.6 ppm, 20 minutes — 8.3 ppm

1/16 LPM flow rate – 100 gamma
10 minutes — 7.4 ppm, 20 minutes — 8.6 ppm

Using 2000ml distilled water we tested the concentration in the water at both 20 and 30 minute increments (we used longer time periods to compensate for the larger volume of water:

1/2 LPM flow rate – 32 gamma
20 minutes — 4.6 ppm, 30 minutes — 5.2 ppm

¼ LPM flow rate – 56 gamma
20 minutes — 5.1 ppm, 30 minutes — 6.6 ppm

⅛ LPM flow rate – 75 gamma
20 minutes — 5.6 ppm, 30 minutes — 7.5 ppm

1/16 LPM flow rate – 100 gamma
20 minutes — 5.4 ppm, 30 minutes — 7.2 ppm

Highly concentrated ozone water is achievable when using the correct oxygen flow rate, ozone concentration, and bubbling system.

We hope this information proves useful. If you have any questions about ozonated water or ozone therapy, please visit our Support page to contact us.